Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO) (HY422056)

Name: Anti-Human CD28 Reference Antibody (Pegrizeprument, RUO)
Brand: abinScience
SKU: HY422056
Price: 370 USD
Availability: InStock

データシート製品共有情報コピー

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100ug
1mg
5mg
10mg
25mg
50mg
100mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY422056

説明

Pegrizeprument (HY422056) is a research-grade recombinant antibody targeting CD28. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

種反応性

Human

アプリケーション

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

宿主種

Chimeric

クローン性

Monoclonal

アイソタイプ

Immunoglobulin Fab VH-G1CH1h_L-kappa

発現システム

Mammalian cells

テスト済みアプリケーション

ターゲット

CD28, TP44, T-cell-specific surface glycoprotein CD28

エンドトキシンレベル

< 10 EU/mg

純度

>95% purity as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P10747

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

別名

2829316-24-5

背景

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475

2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038

3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965

4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202

6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608

7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933

注意事項

For research use only. Not suitable for clinical or therapeutic use.

イメージ

SDS-PAGE

SDS-PAGE for Research Grade Pegrizeprument.
Western blot

Western blot analysis was performed using anti-CD28 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD28 (Catalog No: HY422011)
Lane 2: Negative control