Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO) (HF564016)

Name: Anti-Human B7-H2 Reference Antibody (Prezalumab, RUO)
Brand: abinScience
SKU: HF564016
Price: 176 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HF564016
Descripción	Prezalumab (HF564016) is a research-grade recombinant antibody targeting CD275. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Reactividad de especies	Human
Aplicaciones	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Especie huésped	Human
Clonalidad	Monoclonal
Isotipo	IgG2-kappa
Sistema de expresión	Mammalian cells
Objetivo	B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50
Nivel de endotoxinas	< 10 EU/mg
Pureza	>95% purity as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	O75144
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	AMG557, MEDI-5872, 1523164-68-2
Fondo	ICOS ligand (CD275/ICOSLG) is a ~33 kDa protein. Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion. Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP). 1. Yoshinaga, SK. et al. (2000) International immunology 12, 1439-47. PMID: 11007762 2. Wang, S. et al. (2000) Blood 96, 2808-13. PMID: 11023515 3. Roussel, L. et al. (2018) The Journal of experimental medicine 215, 3151-3164. PMID: 30498080
Nota	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG2 Recombinant Isotype Control Antibody (HyHEL-10) (HV988107)

Imágenes

SEC-HPLC

SEC-HPLC detection for Research Grade Prezalumab.
Bioactivity

Detects CD275/ICOSLG in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Prezalumab.
Flow CytoMetry

Flow-cytometry using anti-human CD275 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD275 monoclonal antibody (Catalog: HF564016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.