Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO) (HY276016)

Name: Anti-Human alpha-Fetoprotein/AFP Reference Antibody (Tacatuzumab, RUO)
Brand: abinScience
SKU: HY276016
Price: 176 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HY276016
説明	Tacatuzumab (HY276016) is a research-grade recombinant antibody targeting Alpha-fetoprotein. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性	Human
アプリケーション	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG1-kappa
発現システム	Mammalian cells
ターゲット	Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
エンドトキシンレベル	< 10 EU/mg
純度	>95% purity as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P02771
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景	Alpha-fetoprotein (AFP) is a ~68 kDa protein. Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
注意事項	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

イメージ

SEC-HPLC

SEC-HPLC detection for Research Grade Tacatuzumab.
Bioactivity

Detects AFP/Alpha-fetoprotein in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Tacatuzumab.
Flow CytoMetry

Flow-cytometry using APC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
Lane 2: negative control
Immunofluorescence

Immunofluorescence staining of HepG2 with Human monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.