Protein A/G purified from cell culture supernatant.
Numéro d'accès
P05112
Forme
Liquid
Tampon de stockage
0.01M PBS pH 7.4.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application
Dilution
WB
1:500-1:2000
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
VAK-694, NVP-VAK-694
Contexte
Interleukin-4 (IL4) is a ~17 kDa protein. Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1. Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.
1. Carr, C. et al. (1991) Biochemistry 30, 1515-23. PMID: 1993171
2. Yokota, T. et al. (1986) Proceedings of the National Academy of Sciences of the United States of America 83, 5894-8. PMID: 3016727
3. Delespesse, G. et al. (1989) Journal of immunology (Baltimore, Md. : 1950) 142, 134-8. PMID: 2521231
4. Hage, T. et al. (1999) Cell 97, 271-81. PMID: 10219247
5. Hülsmeyer, M. et al. (2001) Acta crystallographica. Section D, Biological crystallography 57, 1334-6. PMID: 11526337
6. LaPorte, SL. et al. (2008) Cell 132, 259-72. PMID: 18243101
7. Malabarba, MG. et al. (1995) The Journal of biological chemistry 270, 9630-7. PMID: 7721895
Reférences
1. Koneczny I, et al. IgG4 Autoantibodies in Organ-Specific Autoimmunopathies: Reviewing Class Switching, Antibody-Producing Cells, and Specific Immunotherapies. Front Immunol. 2022;13:834342. Published 2022 Mar 24. [HY457026] 2. Pfaar O, et al. Anti-IgE: A treatment option in allergic rhinitis?. Allergol Select. 2021;5:119-127. Published 2021 Feb 24. [HY457026]
Note
For research use only. Not suitable for clinical or therapeutic use.
Detects recombinant IL4 (Catalog No: HY457021) in indirect ELISA.
Western blot
Western blot analysis was performed using anti-IL4 monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human IL4 (Catalog No: HY457021) Lane 2: Negative control
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