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Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO) (HF996136)

Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)
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概要
カタログ番号HF996136
説明
Vopikitug (HF996136) is a research-grade recombinant antibody targeting CD25. Produced in mammalian cells with native-like glycosylation.
Highlights
  • ●Research Grade — For PK/PD studies, assay development, and ADA research.
  • ●Native Glycosylation — Mammalian expression ensures native-like patterns.
種反応性Human
アプリケーションELISA, Bioactivity: FCM, Functional assay, Research in vivo
宿主種Human
クローン性Monoclonal
アイソタイプIgG1-kappa
テスト済みアプリケーション FCM
ターゲット CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
エンドトキシンレベル < 10 EU/mg
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P01589
形態 Liquid
保存バッファー 0.01M PBS pH 7.4.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名2733581-99-0
背景

Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

注意事項 For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
イメージ
  • Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)

    Flow CytoMetry

    Flow-cytometry using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD25 monoclonal antibody (Catalog: HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)

    Flow cytometry

    Flow-cytometry APC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD25/IL-2R alpha Reference Antibody (Vopikitug, RUO)

    Flow cytometry

    Flow-cytometry PE using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD25 monoclonal antibody (Catalog HF996136) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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