The abinScience Tricolor Prestained Protein Marker is a ready-to-use mixture of nine (9) prestained proteins with apparent molecular weights ranging from 10 to 250 kDa. Three distinct colors — blue, orange, and green — enable rapid band identification on SDS-PAGE gels and easy monitoring of Western blot transfer efficiency. The marker is supplied in a ready-to-use format: simply load directly onto the gel with no heating, reduction, or addition of sample buffer required. Compatible with standard SDS-PAGE, Bis-Tris, Tris-Glycine, and Tris-Acetate gel systems. Detectable by colorimetric, NIR fluorescence (700 nm), and RGB fluorescence (555 nm) methods.
Aplicaciones
SDS-PAGE, Western Blot (WB)
Peso molecular observado
250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
Forma
Liquid
Buffer de almacenamiento
62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% Glycerol
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Ready-to-use prestained protein molecular weight standard for SDS-PAGE and Western blot. Nine color-coded bands allow instant visual identification of molecular weight range during electrophoresis and real-time monitoring of protein transfer to membranes. Three reference bands (70 kDa — orange; 25 kDa — green; others — blue) serve as orientation markers.
Envío
Ship on wet ice or dry ice
Información de uso del producto
Aplicación
Dilución
Load 5 μL per lane for standard mini-gel (0.75–1.0 mm thickness). For large-format gels, use 10–15 μL per lane. Load directly — no heating, reduction, or sample buffer addition required. Apparent molecular weights are determined under standard SDS-PAGE (Tris-Glycine) conditions and may vary slightly depending on gel chemistry and running conditions.
Estabilidad y almacenamiento
Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. Stable for up to 12 months when stored as directed.
Especificaciones
Molecular Weight Bands: 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa (9 bands) Detection Methods: Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) Stain Type: Tricolor (Blue, Orange, Green) Size Range: 10–250 kDa Gel Compatibility: Standard SDS-PAGE, Bis-Tris, Tris-Glycine, Tricine, and Tris-Acetate gel systems Format: Ready-to-use; no heating, reduction, or sample buffer needed Quantity: 2 × 250 μL/pack Applications: SDS-PAGE, Western Blot
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Nombres alternativos
Prestained Protein Ladder, Tricolor Prestained Protein Marker, Pre-stained Protein Marker, Protein Molecular Weight Marker, MW Marker, 预染蛋白Marker
Fondo
Prestained protein markers are indispensable tools in protein electrophoresis and Western blotting workflows. By providing visible molecular weight references directly on the gel and membrane, they eliminate the need for post-run staining of the marker lane and enable real-time monitoring of electrophoretic separation and transfer efficiency. Tricolor markers with distinct chromatic bands further accelerate band identification, reducing the risk of misinterpreting molecular weight estimates. The 10–250 kDa range covers the vast majority of commonly studied proteins, making this marker suitable for routine laboratory use across diverse research applications.
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