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PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
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  • 15KU
  • 75KU
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  • Overview

  • Images

  • References

  • Datasheet

  • SDS

Overview
Catalog No.JN842012
Expression systemE. coli
Protein lengthPNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
ApplicationsRemoval of high mannose N-glycans from glycoproteins
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Form Liquid
Storage buffer 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCAS: 83534-39-8
References 1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012]
2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012]
Note For research use only.
Images
  • PNGase F

    SDS-PAGE

    SDS-PAGE for PNGase F

  • PNGase F

    Bioactivity

    Deglycosylation of Human IgG1 Fc by PNGase F.

  • PNGase F

    Bioactivity

    Schematic diagram of PNGase F cleavage site.

Datasheet
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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