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Tag Antibodies (Epitope-Tag Detection)

abinScience tag antibodies are optimized for detecting epitope-tagged proteins across WB, IP/Co-IP, IF/IHC, and flow cytometry. Covers FLAG, HA, Myc, His, V5, GST, GFP/mCherry. Clone-defined, application-validated, with unconjugated & conjugated formats (HRP/biotin/Alexa/FITC/PE). Lot-specific CoAs. RUO

Why Researchers Choose abinScience

Clone-Defined Monoclonals & polyclonals with epitope sequences
Application-Validated WB, IP, IF, Flow with images
Flexible Formats Unconjugated + HRP/biotin/fluor conjugates
Lot-Specific CoA Purity, storage, reconstitution

Shop by Tag

Selection Guide

  1. Application: Choose high-affinity monoclonals for WB/IP; bright, stable fluor conjugates (AF488/AF647, PE) for IF/Flow.
  2. Epitope Exposure: N/C-terminal placement, linker length, and folding affect recognition. Use polyclonals or switch tags if masked.
  3. Conjugate: HRP/biotin for WB/ELISA; Alexa/FITC/PE for IF/Flow; unconjugated for IP to avoid interference.
  4. Controls: Use positive control plasmids/known expressors; peptide competition for FLAG/HA/Myc; untransfected cells for GFP.
  5. Denaturing vs Native: WB (denaturing) and IP (native) have different antibody requirements. Check datasheet validation.

Applications

Recombinant protein detection and purification (WB, IP/Co-IP)
Cellular localization and live/fixed-cell imaging (IF/IHC)
Flow cytometry detection of tagged surface proteins

Documents & QC

Datasheet with clone, epitope, dilutions, images
Lot-specific CoA: purity, storage, stability

FAQs

Will anti-FLAG/HA/Myc work at either terminus?

Generally yes; extreme proximity to membranes or folded domains may mask epitopes—use flexible linkers (e.g., GGGGS) or switch tag/antibody.

Does anti-His recognize 8×His or 10×His?

Most anti-His detect poly-His lengths ≥6; 8× and 10×His are recognized. Check product page for validation and buffer guidance.

Which conjugate should I choose?

HRP/biotin for WB/ELISA; Alexa 488/647 or PE for IF/Flow; use unconjugated for IP to avoid steric hindrance or background.

RUO disclaimer

All products are for Research Use Only (RUO) and not intended for diagnostic procedures.

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  • Product types
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    Antibodies (72)
  • Target
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    GST tag (4)
    Softag1 epitope tag (1)
    CTNNB1 (1)
    FITC/Fluorescein isothiocyanate (4)
    GCN4 tag (1)
    MBP Tag (2)
    Protein C tag (1)
    His Tag (3)
    Ganglioside GM1 (1)
    Strep Tag (3)
    SUMO-tag (3)
    c-Myc (1)
    DLVPR Epitope Tag (1)
    Twin-Strep tag (1)
    PA tag (1)
    6*His Tag (5)
    mTagBFP (1)
    Enhanced GFP (1)
    GFP (8)
    EPEATag (1)
    BC2 tag (1)
    EE epitope tag (1)
    APP/Amyloid beta (1)
    Flag tag (3)
    Strep Tag (WSHPQFEK) (2)
    mCherry (5)
    HA Tag (2)
    BRIL/cybC (1)
    EGFP (2)
    Biotin (2)
    dsRed (1)
    Avi tag (4)
    ALFA tag (1)
    V5 Tag (2)
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    Polyclonal (3)
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  • Conjugate
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    HRP (8)
    APC (1)
    PE (1)
    Unconjugated (60)
    FITC (1)

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