Assay Kits
abinScience assay kits unify ELISA, ADA, and PK workflows for biomarker quantitation and biotherapeutics research. Each lot ships with verified sensitivity (LoD/LoQ), dynamic range, linearity, precision and spike-recovery. Protocols are optimized for serum/plasma (EDTA/heparin/citrate) with MRD guidance. Drug-tolerant options support ADA testing, while anti-idiotype capture enables specific PK quantitation of free or total drug. Ready-to-run reagents, matched controls and calibrators streamline reproducible results. RUO
Why Researchers Choose abinScience
Lot-Specific CoA LoD/LoQ, linearity, precision, spike-recovery
Ready-to-Run Reagents with matched controls & calibrators
Matrix Compatibility Serum/plasma with clear MRD/matrix guidance
Flexible Throughput 96-well strip plates for partial runs
Specialized Workflows Drug-tolerant ADA; anti-idiotype PK specificity
Selection Guide
ELISA: Sandwich vs. Competitive
Sandwich ELISA for larger proteins with two epitopes; competitive ELISA for small molecules or single accessible epitopes. Choose colorimetric (OD450), chemiluminescent or fluorescent readout per instrument and expected range.
ADA: Tiered Workflow & Drug Tolerance
Implement screening → confirmatory (specificity) → titer. Use drug-tolerant options (e.g., acid dissociation) to mitigate circulating-drug interference; follow cut-point and acceptance criteria in the kit guide.
PK: Free vs. Total Drug Quantitation
Free-drug assays measure unbound drug; total-drug assays quantify both free and complexed forms. Anti-idiotype reagents enhance specificity; evaluate dilution linearity across the reportable range.
Matrix, MRD & Range
Match expected concentrations to the kit’s dynamic range; apply recommended MRD to minimize matrix effects in serum/plasma. Review spike-recovery and precision on the lot-specific CoA.
Applications
Biomarker & cytokine quantitation (ELISA)
Biotherapeutics immunogenicity assessment (ADA)
Drug exposure monitoring & comparability (PK)
Documents & QC
Standard curve examples, acceptance criteria & worksheets
Storage/stability, MRD, interference guidance (ADA/PK)
Lot tracking & ordering info
FAQs
When should I choose sandwich vs. competitive ELISA?
Sandwich ELISA suits larger proteins with two non-overlapping epitopes; competitive ELISA fits small molecules or single-epitope targets.
What is a tiered ADA workflow?
Samples undergo screening for binding ADA, confirmatory testing for specificity, and titering to rank response magnitude.
How do you address drug interference in ADA?
Use drug-tolerant approaches (e.g., acid dissociation) and optimized MRD; follow the kit’s cut-point and acceptance criteria.
PK: difference between free and total drug assays?
Free-drug assays quantify unbound drug; total-drug assays capture both free and complexed forms, supporting exposure assessment.
What do LoD and LoQ mean?
LoD is the lowest detectable concentration above blank; LoQ is the lowest level meeting precision/accuracy criteria.
Which matrices are supported?
Serum and plasma (EDTA/heparin/citrate) with matrix-specific dilution guidance; see the lot-specific CoA.
RUO disclaimer
All assay kits are for Research Use Only and not for diagnostic procedures.
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