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Prestained Protein Marker (10–250 kDa) (PM100018)

Prestained Protein Marker (10–250 kDa)
Prestained Protein Marker (10–250 kDa)
価格(USD): --
スペック:
  • 250ul
数量:
お問い合わせ
  • 概要

  • イメージ

  • 参考文献

  • データシート

  • SDS

概要
カタログ番号PM100018
ブランドabinScience
説明

The abinScience Tricolor Prestained Protein Marker is a ready-to-use mixture of nine (9) prestained proteins with apparent molecular weights ranging from 10 to 250 kDa. Three distinct colors — blue, orange, and green — enable rapid band identification on SDS-PAGE gels and easy monitoring of Western blot transfer efficiency. The marker is supplied in a ready-to-use format: simply load directly onto the gel with no heating, reduction, or addition of sample buffer required. Compatible with standard SDS-PAGE, Bis-Tris, Tris-Glycine, and Tris-Acetate gel systems. Detectable by colorimetric, NIR fluorescence (700 nm), and RGB fluorescence (555 nm) methods.

アプリケーションSDS-PAGE, Western Blot (WB)
観測分子量 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
形態 Liquid
保存バッファー 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% Glycerol

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

検出方法 Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
特性 Ready-to-use prestained protein molecular weight standard for SDS-PAGE and Western blot. Nine color-coded bands allow instant visual identification of molecular weight range during electrophoresis and real-time monitoring of protein transfer to membranes. Three reference bands (70 kDa — orange; 25 kDa — green; others — blue) serve as orientation markers.
発送 Ship on wet ice or dry ice
製品使用情報
アプリケーション 希釈
Load 5 μL per lane for standard mini-gel (0.75–1.0 mm thickness). For large-format gels, use 10–15 μL per lane. Load directly — no heating, reduction, or sample buffer addition required. Apparent molecular weights are determined under standard SDS-PAGE (Tris-Glycine) conditions and may vary slightly depending on gel chemistry and running conditions.
安定性と保存 Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. Stable for up to 12 months when stored as directed.
仕様

Molecular Weight Bands: 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa (9 bands) Detection Methods: Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) Stain Type: Tricolor (Blue, Orange, Green) Size Range: 10–250 kDa Gel Compatibility: Standard SDS-PAGE, Bis-Tris, Tris-Glycine, Tricine, and Tris-Acetate gel systems Format: Ready-to-use; no heating, reduction, or sample buffer needed Quantity: 2 × 250 μL/pack Applications: SDS-PAGE, Western Blot

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別名Prestained Protein Ladder, Tricolor Prestained Protein Marker, Pre-stained Protein Marker, Protein Molecular Weight Marker, MW Marker, 预染蛋白Marker
背景

Prestained protein markers are indispensable tools in protein electrophoresis and Western blotting workflows. By providing visible molecular weight references directly on the gel and membrane, they eliminate the need for post-run staining of the marker lane and enable real-time monitoring of electrophoretic separation and transfer efficiency. Tricolor markers with distinct chromatic bands further accelerate band identification, reducing the risk of misinterpreting molecular weight estimates. The 10–250 kDa range covers the vast majority of commonly studied proteins, making this marker suitable for routine laboratory use across diverse research applications.

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イメージ
  • Prestained Protein Marker (10–250 kDa)

    SDS-PAGE

    SDS-PAGE band profile

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
レコメンデーション

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