Anti-Human DNAJB11 Polyclonal Antibody (HP182014)

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개요
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개요

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HP182014

설명

Anti-Human DNAJB11 Polyclonal Antibody (HP182014) is a rabbit polyclonal antibody detecting DNAJB11 in ELISA, IHC, WB. Suitable for Bovine, Dog, Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Bos taurus, Dog, Human, Mouse, Pongo abelii, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human DNAJB11 (Gly23-Glu333).

타겟

APOBEC1-binding protein 2, DNAJB11, DnaJ homolog subfamily B member 11, ERj3p, PWP1-interacting protein 4, ER-resident protein ERdj3, ABBP-2, DnaJ protein homolog 9, EDJ, ERJ3, Human DnaJ protein 9, HDJ9, HEDJ, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, ERdj3, Endoplasmic reticulum DNA J domain-containing protein 3, hDj-9

정제

Purified by antigen affinity column.

등록번호

Q9UBS4

RRID

Anti-Human DNAJB11 Polyclonal Antibody (abinScience Cat# HP182014, RRID:AB_3730666)

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DnaJ homolog subfamily B member 11 (DNAJB11) is a ~40 kDa protein. As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.

1. Yu, M. et al. (2000) The Journal of biological chemistry 275, 24984-92. PMID: 10827079

2. Shen, Y. et al. (2005) Molecular biology of the cell 16, 40-50. PMID: 15525676

3. Cornec-Le Gall, E. et al. (2018) American journal of human genetics 102, 832-844. PMID: 29706351

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-DNAJB11 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raji cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A549 cell lysate
Lane 5: 293T cell lysate
Lane 6: MCF7 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: Mouse heart lysate
Lane 9: Mouse liver lysate
Lane 10: Mouse lung lysate
Lane11: Rat heart lysate
Lane12: Rat liver lysate
Lane13: Rat lung lysate

참고 문헌

데이터시트

Anti-Human DNAJB11 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human DNAJB11 Polyclonal Antibody (HP182014)

개요

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