Immune Checkpoint Proteins

Q: What does “carrier-free” mean?

Carrier-free proteins contain no stabilizers (e.g., BSA), reducing interference in sensitive assays and enabling precise mass-based dosing. Ideal for SPR, BLI, and cell-based functional studies.

Q: Which expression system should I choose?

HEK293/CHO: Native folding, glycosylation, and disulfide bonds — ideal for receptor-binding and functional studies.E. coli: High yield and cost-effective, best for structural screening.Insect/Yeast: Balanced PTMs with good scalability.

Q: What endotoxin level is recommended for immune cell assays?

≤0.1 EU/µg is the standard for immune cell culture, T-cell expansion, and in vivo studies. Every lot ships with LAL-verified endotoxin data on the CoA.

Q: How to reconstitute lyophilized proteins?

Use sterile, endotoxin-free buffer. Gently swirl — avoid vortexing. Aliquot into single-use volumes and store at -80°C to prevent freeze-thaw damage.

Q: Are these proteins RUO or GMP-grade?

Unless explicitly labeled, all products are Research Use Only (RUO). For GMP-grade requirements, contact our team for custom manufacturing options.

Browse recombinant immune checkpoint proteins — PD-1, PD-L1, CTLA-4, LAG-3, TIGIT, TIM-3, B7-H3, and more. Produced in HEK293/CHO for native glycosylation. Available as His-tagged, Fc-chimera, and biotinylated formats for bioassay development, SPR binding, and therapeutic screening.RUO

연구자들이 abinScience를 선택하는 이유

≥95% 순도 및 낮은 내독소 수준 Typical ≤0.1 EU/µg verified by LAL assay. Lot-specific CoA with SDS-PAGE and SEC-HPLC data included.

생물학적 활성 검증됨 Functional validation via ELISA or cell-based proliferation/signaling assay. High batch-to-batch consistency (<20% CV).

5가지 발현 시스템 HEK293 & CHO for native PTMs; E. coli for high-yield screening; insect and yeast for balanced cost and glycosylation.

유연한 형식 Carrier-free (no BSA), His-tagged, Fc-chimera, biotinylated; lyophilized or liquid — choose the best fit for your assay.

확장성 및 신뢰성 From µg to mg quantities with consistent lead times. Global cold-chain shipping with dry-ice packaging.

올바른 제품을 선택하는 방법

응용 분야:

Choose carrier-free for sensitive bioassays (e.g., CAR-T expansion, organoid culture, SPR). His-tag works best for pull-down and purification workflows.

발현 시스템:

Use HEK293/CHO for native glycosylation and disulfide bonds; E. coli for cost-effective high-throughput screening; insect/yeast when moderate PTMs are sufficient.

태그 및 형식:

His-tag for IMAC purification and binding studies; Fc-chimera for enhanced avidity and half-life; biotinylated for immobilization on streptavidin surfaces.

내독소:

Match your assay tolerance — ≤0.1 EU/µg is recommended for immune cell culture and in vivo studies. Always verify via the lot-specific CoA.

문서 및 QC 데이터

Download Certificate of Analysis (CoA), SDS-PAGE gels, SEC-HPLC chromatograms, and reconstitution protocols on each product page. 모든 QC 데이터는 특정 로트(lot)에 한정되며 추적성을 위해 보관됩니다.

자주 묻는 질문(FAQ)

"캐리어 프리(carrier-free)"란 무엇을 의미하나요?

Carrier-free proteins contain no stabilizers (e.g., BSA), reducing interference in sensitive assays and enabling precise mass-based dosing. Ideal for SPR, BLI, and cell-based functional studies.

어떤 발현 시스템을 선택해야 하나요?

HEK293/CHO: Native folding, glycosylation, and disulfide bonds — ideal for receptor-binding and functional studies.
E. coli: High yield and cost-effective, best for structural screening.
Insect/Yeast: Balanced PTMs with good scalability.

면역 세포 분석에 권장되는 내독소 수준은 얼마인가요?

≤0.1 EU/µg is the standard for immune cell culture, T-cell expansion, and in vivo studies. Every lot ships with LAL-verified endotoxin data on the CoA.

동결건조된 단백질을 재구성하는 방법은 무엇인가요?

Use sterile, endotoxin-free buffer. Gently swirl — avoid vortexing. Aliquot into single-use volumes and store at -80°C to prevent freeze-thaw damage.

이 단백질들은 연구용(RUO) 등급인가요, 아니면 GMP 등급인가요?

Unless explicitly labeled, all products are Research Use Only (RUO). For GMP-grade requirements, contact our team for custom manufacturing options.

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