Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

Name: Anti-Human LYPLA1 Polyclonal Antibody
Brand: abinScience
SKU: HF852014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호

HF852014

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human LYPLA1 (Met1-Asp230).

타겟

APT1, LYPLA1, LPL-I, Lysophospholipase I, LysoPLA I, LPL1, hAPT1, Acyl-protein thioesterase 1, APT-1, Lysophospholipase 1

정제

Purified by antigen affinity column.

등록번호

O75608

RRID

Anti-Human LYPLA1 Polyclonal Antibody (abinScience Cat# HF852014, RRID:AB_3731140)

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Acyl-protein thioesterase 1 (LYPLA1) is a ~24 kDa protein. Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC).

1. Hirano, T. et al. (2009) Biochimica et biophysica acta 1791, 797-805. PMID: 19439193

2. Dekker, FJ. et al. (2010) Nature chemical biology 6, 449-56. PMID: 20418879

3. Tian, L. et al. (2012) The Journal of biological chemistry 287, 14718-25. PMID: 22399288

4. Adachi, N. et al. (2016) The Journal of biological chemistry 291, 20232-46. PMID: 27481942

5. Huang, X. et al. (2023) Molecular cell 83, 3485-3501.e11. PMID: 37802024

6. Bolen, AL. et al. (2011) Journal of lipid research 52, 958-70. PMID: 21393252

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-LYPLA1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate
Lane 2: Mouse kidney lysate
Lane 3: Rat liver lysate
Lane 4: Rat kidney lysate

참고 문헌

데이터시트

Anti-Human LYPLA1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human LYPLA1 Polyclonal Antibody (HF852014)

개요

이미지

참고 문헌

데이터시트

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