Anti-Mouse TP53/p53 Polyclonal Antibody (MY039014)

데이터시트 제품 공유 정보 복사

가격(USD): $

사양:

20ug
50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호

MY039014

설명

Anti-Mouse TP53/p53 Polyclonal Antibody (MY039014) is a rabbit polyclonal antibody detecting TP53 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Mouse TP53/p53 (Met1-Asp390).

타겟

Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53

정제

Purified by antigen affinity column.

등록번호

P02340

RRID

Anti-Mouse TP53/p53 Polyclonal Antibody (abinScience Cat# MY039014, RRID:AB_3723888)

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

1. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538

2. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990

3. Vaseva, AV. et al. (2012) Cell 149, 1536-48. PMID: 22726440

4. Miki, T. et al. (2013) Nature communications 4, 2444. PMID: 24051492

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-TP53 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HT-29 cell lysate
Lane 2: 293T cell lysate
Lane 3: Raji cell lysate