Anti-HDAC1 Polyclonal Antibody (HC626024)

데이터시트 제품 공유 정보 복사

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사양:

50ug
100ug

수량:

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개요
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참고 문헌
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개요

카탈로그 번호

HC626024

설명

Anti-HDAC1 Polyclonal Antibody (HC626024) is a rabbit polyclonal antibody detecting HDAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human HDAC1 (Met1-Ala482).

타겟

Histone deacetylase 1, HDAC1, RPD3L1, HD1

정제

Purified by antigen affinity column.

등록번호

Q13547

RRID

Anti-HDAC1 Polyclonal Antibody (abinScience Cat# HC626024, RRID:AB_3724067)

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Histone deacetylase 1 (HDAC1) is a ~55 kDa protein. Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription.

1. Qiu, Y. et al. (2006) Molecular cell 22, 669-79. PMID: 16762839

2. Wang, C. et al. (2007) The Journal of biological chemistry 282, 29902-9. PMID: 17704056

3. Wei, W. et al. (2017) Cell research 27, 898-915. PMID: 28497810

4. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

5. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

6. Jelinic, P. et al. (2011) Molecular and cellular biology 31, 54-62. PMID: 21041482

8. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960

9. Zheng, G. et al. (2006) Biochemical and biophysical research communications 339, 1069-75. PMID: 16337145

10. Hung, JJ. et al. (2006) Molecular and cellular biology 26, 1770-85. PMID: 16478997

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-HDAC1 polyclonal antibody at 1ug/mL on recombinant HDAC1 (Catalog No. HC626022).

참고 문헌

데이터시트

Anti-HDAC1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-HDAC1 Polyclonal Antibody (HC626024)

개요

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참고 문헌

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