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| カタログ番号 | JN009016 |
|---|---|
| 説明 | |
| 種反応性 | Mycobacterium tuberculosis |
| アプリケーション | ELISA, FCM, Functional assay |
| クローン性 | Monoclonal |
| アイソタイプ | esxA |
| 発現システム | Mammalian cells |
| 性質 | Recombinant |
| ターゲット | 6 kDa early secretory antigenic target, ESAT-6, esaT6, esxA |
| エンドトキシンレベル | < 10 EU/mg as determined by LAL method. |
| 純度 | >95% as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P9WNK7 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term from the date of receipt.. |
| 別名 | CAS: 3084143-81-4 |
| 背景 | 6 kDa early secretory antigenic target (esxA/ESAT-6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis. 1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590 2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876 3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536 4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219 5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145 6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573 7. Pathak, SK. et al. (2007) Nature immunology 8, 610-8. PMID: 17486091 8. Mishra, BB. et al. (2010) Cellular microbiology 12, 1046-63. PMID: 20148899 9. Boggaram, V. et al. (2013) The Journal of biological chemistry 288, 25500-25511. PMID: 23867456 10. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553 |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
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