Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (SAA2172) (JN009013)

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Overview

Catalog No.

JN009013

Description

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (SAA2172) [SAA2172] (JN009013) is a human monoclonal antibody detecting ESAT-6 in ELISA.

Species reactivity

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Applications

ELISA

Host species

Human

Isotype

IgG1

Clone ID

SAA2172

Clonality

Monoclonal

Target

6 kDa early secretory antigenic target, ESAT-6, esxA, esaT6, Rv3875, MTV027.10

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P9WNK7

RRID

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (SAA2172) (abinScience Cat# JN009013, RRID:AB_3729538)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

6 kDa early secretory antigenic target (esxA/ESAT-6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.

1. Renshaw, PS. et al. (2002) The Journal of biological chemistry 277, 21598-603. PMID: 11940590

2. Sørensen, AL. et al. (1995) Infection and immunity 63, 1710-7. PMID: 7729876

3. Stanley, SA. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 13001-6. PMID: 14557536

4. Andersen, P. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 3359-72. PMID: 7897219

5. Wang, X. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 3668-77. PMID: 19265145

6. Peng, H. et al. (2011) The Journal of biological chemistry 286, 24508-18. PMID: 21586573

7. Pathak, SK. et al. (2007) Nature immunology 8, 610-8. PMID: 17486091

8. Mishra, BB. et al. (2010) Cellular microbiology 12, 1046-63. PMID: 20148899

9. Boggaram, V. et al. (2013) The Journal of biological chemistry 288, 25500-25511. PMID: 23867456

10. Sreejit, G. et al. (2014) PLoS pathogens 10, e1004446. PMID: 25356553

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mycobacterium tuberculosis esxA/ESAT-6 Antibody (SAA2172)

References

Datasheet

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (SAA2172).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mycobacterium tuberculosis esxA/ESAT-6 Recombinant Antibody (SAA2172) (JN009013)

Overview

Images

References

Datasheet

SDS

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