Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013)

Name: Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039)
Brand: abinScience
SKU: HB644013
Price: 191 USD
Availability: InStock

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100ul

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Número de catálogo	HB644013
Descripción	Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013) is a rabbit monoclonal antibody detecting L-Selectin/CD62L in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Reactividad de especies	Human
Aplicaciones	IF, FCM
Especie huésped	Rabbit
Clonalidad	Monoclonal
Isotipo	IgG
ID de clon	SRR0039
Aplicaciones probadas	IF, FCM
Objetivo	CD62 antigen-like family member L, CD62L, L-selectin, LAM-1, LECAM1, LNHR, LYAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
Nivel de endotoxinas	< 10 EU/mg as determined by LAL method.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P14151
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo	L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. 1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782 2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641 3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325
Nota	For research use only.

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Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting L-Selectin/CD62L (HB644013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of L-Selectin/CD62L was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD62L antibody. Loucy cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD62L monoclonal antibody (Catalog HB644013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039).pdf

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Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013)

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