Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013)

Name: Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039)
Brand: abinScience
SKU: HB644013
Price: 191 USD
Availability: InStock

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50ul
100ul

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Каталожный номер	HB644013
Описание	Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013) is a rabbit monoclonal antibody detecting L-Selectin/CD62L in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Реактивность видов	Human
Применения	IF, FCM
Хозяинский вид	Rabbit
Клоональность	Monoclonal
Изотип	IgG
ID клона	SRR0039
Проверенные приложения	IF, FCM
Цель	CD62 antigen-like family member L, CD62L, L-selectin, LAM-1, LECAM1, LNHR, LYAM1, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
Уровень эндотоксинов	< 10 EU/mg as determined by LAL method.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P14151
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон	L-selectin (CD62L/SELL) is a ~42 kDa protein. Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. 1. Bernimoulin, MP. et al. (2003) The Journal of biological chemistry 278, 37-47. PMID: 12403782 2. Mehta-D'souza, P. et al. (2017) The Journal of biological chemistry 292, 2510-2518. PMID: 28011641 3. Wedepohl, S. et al. (2017) Chembiochem : a European journal of chemical biology 18, 1338-1345. PMID: 28489325
Примечание	For research use only.

Изображения

Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting L-Selectin/CD62L (HB644013) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of L-Selectin/CD62L was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD62L antibody. Loucy cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD62L monoclonal antibody (Catalog HB644013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039).pdf

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Anti-Human L-Selectin/CD62L Recombinant Antibody (SRR0039) (HB644013)

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