Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043)

Name: Anti-Human B-Raf Recombinant Antibody (SRR0002)
Brand: abinScience
SKU: HB617043
Price: 191 USD
Availability: InStock

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50ul
100ul

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Número de catálogo	HB617043
Descripción	Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043) is a rabbit monoclonal antibody detecting B-Raf in IF, WB. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Reactividad de especies	Human
Aplicaciones	IF, WB
Especie huésped	Rabbit
Clonalidad	Monoclonal
Isotipo	IgG
ID de clon	SRR0002
Aplicaciones probadas	IF, WB
Objetivo	BRAF, BRAF1, EC:2.7.11.1, Proto-oncogene B-Raf, RAFB1, Serine/threonine-protein kinase B-raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Nivel de endotoxinas	< 10 EU/mg as determined by LAL method.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P15056
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo	Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons. 1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910 2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126 3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789 4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179
Nota	For research use only.

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Western blot

Western blot analysis was performed using anti-B-Raf monoclonal antibody at 1 ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HEK293T cell lysate
Immunofluorescence

HeLa cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting B-Raf (HB617043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of B-Raf was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Referencias

Datasheet

Anti-Human B-Raf Recombinant Antibody (SRR0002).pdf

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Anti-Human B-Raf Recombinant Antibody (SRR0002) (HB617043)

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Datasheet

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