Anti-Human B-Raf (V600E) Recombinant Antibody (SAA2181) (HB617033)

Name: Anti-Human B-Raf (V600E) Recombinant Antibody (SAA2181)
Brand: abinScience
SKU: HB617033
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB617033

Description

Anti-Human B-Raf (V600E) Recombinant Antibody (SAA2181) (HB617033) is a rabbit monoclonal antibody detecting B-Raf in ELISA. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.

Species reactivity

Human

Applications

ELISA

Host species

Rabbit

Clonality

Monoclonal

Isotype

IgG

Clone ID

SAA2181

Target

BRAF1, RAFB1, Proto-oncogene B-Raf, BRAF, v-Raf murine sarcoma viral oncogene homolog B1, p94, Serine/threonine-protein kinase B-raf

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P15056

RRID

Anti-Human BRAF/B-Raf (V600E) Antibody (SAA2181) (abinScience Cat# HB617033, RRID:AB_3729545)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Serine/threonine-protein kinase B-raf (BRAF) is a ~84 kDa protein. Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. Phosphorylates PFKFB2. May play a role in the postsynaptic responses of hippocampal neurons.

1. Brennan, DF. et al. (2011) Nature 472, 366-9. PMID: 21441910

2. Lavoie, H. et al. (2018) Nature 554, 549-553. PMID: 29433126

3. McGrail, K. et al. (2022) Nature communications 13, 7113. PMID: 36402789

4. Stephens, RM. et al. (1992) Molecular and cellular biology 12, 3733-42. PMID: 1508179

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human BRAF/B-Raf (V600E) Antibody (SAA2181)

References

Datasheet

Anti-Human B-Raf (V600E) Recombinant Antibody (SAA2181).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human B-Raf (V600E) Recombinant Antibody (SAA2181) (HB617033)

Overview

Images

References

Datasheet

SDS