Anti-Human TNF-alpha Recombinant Antibody (SRR0271) (HF879173)

Name: Anti-Human TNF-alpha Recombinant Antibody (SRR0271)
Brand: abinScience
SKU: HF879173
Price: 191 USD
Availability: InStock

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Numéro de catalogue	HF879173
Description	Anti-Human TNF-alpha Recombinant Antibody (SRR0271) (HF879173) is a rabbit monoclonal antibody detecting TNF-alpha in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Reactivité des espèces	Human
Applications	IF, FCM
Espèce hôte	Rabbit
Clonalité	Monoclonal
Isotype	IgG
ID de clone	SRR0271
Applications testées	IF, FCM
Cible	C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
Niveau d'endotoxines	< 10 EU/mg as determined by LAL method.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P01375
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	Tumor necrosis factor (TNFA) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. 1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208 2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952 3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918 4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819
Note	For research use only.

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Immunofluorescence

TNFα-overexpressing cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting TNF-alpha (HF879173) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of TNF-alpha was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human TNFa antibody. Overexpression cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human TNFa monoclonal antibody (Catalog HF879173, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

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Anti-Human TNF-alpha Recombinant Antibody (SRR0271).pdf

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Anti-Human TNF-alpha Recombinant Antibody (SRR0271) (HF879173)

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