Anti-Human TNF-alpha Reference Antibody (Afelimomab, RUO) (HF879056)

Name: Anti-Human TNF-alpha Reference Antibody (Afelimomab, RUO)
Brand: abinScience
SKU: HF879056
Price: 176 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HF879056
Description	Research Grade Afelimomab (HF879056) is a mouse monoclonal antibody detecting TNF in ELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Mouse
Isotype	F(ab')2-G3-kappa
Expression system	Mammalian cells
Species	Human
Clonality	Monoclonal
Target	TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01375
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MAK-195F, 156227-98-4
Background	Tumor necrosis factor (TNFA) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. 1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208 2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952 3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918 4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Afelimomab.
Western blot

Western blot analysis was performed using anti-TNFa monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human TNFa (Catalog No: HF879011)
Lane 2: negative control
Bioactivity

Detects recombinant human TNFa/TNF-alpha (Catalog No: HF879011) in indirect ELISA