Anti-ANXA2 Polyclonal Antibody (HY297014)

데이터시트 제품 공유 정보 복사

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20ug
50ug
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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호

HY297014

설명

Anti-ANXA2 Polyclonal Antibody (HY297014) is a rabbit polyclonal antibody detecting ANXA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Dog, Rat, Bos taurus, Cervus elaphus, Chicken, Ovis aries, Pongo abelii, Pig

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human ANXA2 (Met1-Asp339).

타겟

Lipocortin II, Annexin A2, Calpactin-1 heavy chain, PAP-IV, p36, CAL1H, Calpactin I heavy chain, ANX2, ANX2L4, Protein I, Annexin-2, LPC2D, Annexin II, ANXA2, Placental anticoagulant protein IV, Chromobindin-8

정제

Purified by antigen affinity column.

등록번호

P07355

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Annexin A2 (ANXA2) is a ~38 kDa protein. Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation.

1. Mayer, G. et al. (2008) The Journal of biological chemistry 283, 31791-801. PMID: 18799458

2. Seidah, NG. et al. (2012) PloS one 7, e41865. PMID: 22848640

3. Ly, K. et al. (2014) The Journal of biological chemistry 289, 17732-46. PMID: 24808179

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-ANXA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: PC-12 cell lysate