Anti-CD10/MME Polyclonal Antibody (HY401014)

데이터시트 제품 공유 정보 복사

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사양:

50ug
100ug

수량:

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개요
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개요

카탈로그 번호

HY401014

설명

Anti-CD10/MME Polyclonal Antibody (HY401014) is a rabbit polyclonal antibody detecting CD10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Pongo abelii, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat, Oryctolagus cuniculus, Pongo abelii, Pig

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human CD10/MME (Met1-Cys80).

타겟

Atriopeptidase, Common acute lymphocytic leukemia antigen, Neutral endopeptidase, Skin fibroblast elastase, CD10, SFE, CALLA, Neprilysin, Neutral endopeptidase 24.11, MME, NEP, Enkephalinase, EPN

정제

Purified by antigen affinity column.

등록번호

P08473

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Neprilysin (CD10/MME) is a ~85 kDa protein. Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)).

1. Rice, GI. et al. (2004) The Biochemical journal 383, 45-51. PMID: 15283675

2. Skidgel, RA. et al. (1984) Peptides 5, 769-76. PMID: 6208535

3. Gafford, JT. et al. (1983) Biochemistry 22, 3265-71. PMID: 6349683

4. Le Moual, H. et al. (1994) European journal of biochemistry 221, 475-80. PMID: 8168535

5. Wisner, A. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 17979-84. PMID: 17101991

6. Brandt, I. et al. (2006) Clinical chemistry 52, 82-7. PMID: 16254193

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CD10/MME polyclonal antibody at 1ug/mL on recombinant CD10/MME (Catalog No. HY401012).

참고 문헌

데이터시트

Anti-CD10/MME Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CD10/MME Polyclonal Antibody (HY401014)

개요

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참고 문헌

데이터시트

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