Anti-BDNF Polyclonal Antibody (HB935014)

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개요
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개요

카탈로그 번호

HB935014

설명

Anti-BDNF Polyclonal Antibody (HB935014) is a rabbit polyclonal antibody detecting BDNF in ELISA, IF, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Ailurus fulgens.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Dog, Cat, Acrantophis dumerili, Acrochordus javanicus, Ailuropoda melanoleuca, Ailurus fulgens, Anilius scytale, Aspidites melanocephalus, Boa constrictor, Bos taurus, Calabaria reinhardtii, Candoia carinata, Cavia porcellus, Charina bottae, Chilabothrus striatus, Corallus caninus, Cylindrophis ruffus, Cyprinus carpio, Epicrates cenchria, Equus caballus, Eryx colubrinus colubrinus, Eryx conicus, Eryx jayakari, Eryx johnii, Eunectes notaeus, Exiliboa placata, Chicken, Helarctos malayanus, Lichanura trivirgata, Lipotes vexillifer, Loxocemus bicolor, Macaca mulatta, Macrovipera lebetinus, Morelia spilota, Pan troglodytes, Procyon lotor, Ptyas major, Ramphotyphlops sp., Rat, Sanzinia madagascariensis, Spermophilus citellus, Pig, Tropidophis haetianus, Ursus arctos, Ursus thibetanus, Xenopeltis unicolor, Xenopus laevis, Xiphophorus maculatus

응용 분야

ELISA, IF, IHC, WB

숙주 종

Rabbit

이소타입

IgG

클론성

Polyclonal

면역원

E. coli - derived recombinant Human BDNF (Arg128-Arg247).

타겟

ProBDNF, Abrineurin, Brain-derived neurotrophic factor, BDNF

정제

Purified by antigen affinity column.

등록번호

P23560

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Neurotrophic factor BDNF precursor form is a ~27 kDa protein. Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

1. Mowla, SJ. et al. (2001) The Journal of biological chemistry 276, 12660-6. PMID: 11152678

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-BDNF polyclonal antibody at 1µg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse cerebellum lysate

참고 문헌

데이터시트

Anti-BDNF Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-BDNF Polyclonal Antibody (HB935014)

개요

이미지

참고 문헌

데이터시트

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