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| 카탈로그 번호 | HY286107 |
|---|---|
| 설명 |
Anti-Human CD340/ERBB2/HER2 Antibody (4D5V8) [4D5V8] (HY286107) is a human monoclonal antibody detecting ERBB2 in FCM. Suitable for Human.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | FCM |
| 숙주 종 | Human |
| 이소타입 | IgG1, kappa |
| 클론 ID | 4D5V8 |
| 클론성 | Monoclonal |
| 타겟 | p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P04626 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS, pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 배경 | Receptor tyrosine-protein kinase erbB-2 (CD340/ERBB2) is a ~137 kDa protein. Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. CD340 is the therapeutic target of trastuzumab (Herceptin) and trastuzumab deruxtecan (Enhertu). |
| 참고사항 | For research use only. |
Flow-cytometry using anti-human CD340 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD340 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD340 antibody.SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 antibody monoclonal antibody (Catalog # HY286107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SEC-HPLC detection for Anti-Human CD340/ERBB2/HER2 Antibody (4D5V8).
Flow-cytometry using anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog HY286107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CD340 antibody. MCF-7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD340 monoclonal antibody (Catalog HY286107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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