Anti-Human CD47/MER6 Antibody (5F9-G4) (HC359107)

데이터시트 제품 공유 정보 복사

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100ug

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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호	HC359107
설명	Anti-Human CD47/MER6 Antibody (5F9-G4) [5F9-G4] (HC359107) is a human monoclonal antibody detecting Protein MER6 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
종 반응성	Human
응용 분야	FCM
숙주 종	Human
이소타입	IgG4, kappa
클론 ID	5F9-G4
클론성	Monoclonal
타겟	Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	Q08722
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경	Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594 2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453 3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835 4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831 5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562 6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764
참고사항	For research use only.

이미지

Bioactivity

SEC-HPLC detection for Anti-Human CD47/MER6 Antibody (5F9-G4).
Flow Cytometry

Flow-cytometry using anti-human CD47 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD47 monoclonal antibody (Catalog HC359107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.