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Anti-Human CD80/B7-1 Antibody (SAA2207), FITC (HW630217)

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Anti-Human CD80/B7-1 Antibody (SAA2207), FITC

Anti-Human CD80/B7-1 Antibody (SAA2207), FITC

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100T

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개요
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참고 문헌
데이터시트

개요

카탈로그 번호	HW630217
설명	Anti-Human CD80/B7-1 Antibody (SAA2207), FITC [SAA2207] (HW630217) is a human monoclonal antibody detecting CD80 in ELISA, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
종 반응성	Human
응용 분야	ELISA, FCM
숙주 종	Human
이소타입	IgG1, kappa
클론 ID	SAA2207
접합	FITC
클론성	Monoclonal
타겟	CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1
내독소 수준	Please contact with the lab for this information.
정제	Affinity chromatography.
등록번호	P33681
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Store at 4°C for 12 months. Protect from light. Do not freeze.
배경	T-lymphocyte activation antigen CD80 (B7-1) is a ~33 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines. Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming. In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway. 1. Lu, B. et al. (2024) Acta pharmacologica Sinica 45, 1214-1223. PMID: 38467718 2. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 3. Boulougouris, G. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1809-16. PMID: 10438913 4. Vandenborre, K. et al. (1999) Immunology 98, 413-21. PMID: 10583602 5. Stamper, CC. et al. (2001) Nature 410, 608-11. PMID: 11279502 6. Frauwirth, KA. et al. (2002) Immunity 16, 769-77. PMID: 12121659 7. Kennedy, A. et al. (2023) The EMBO journal 42, e111556. PMID: 36727298
참고사항	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

이미지

Flow Cytometry

Flow-cytometry using FITC anti-Human CD80 antibody. Raji cells were stained with an irrelevant antibody (Red Histogram) or a FITC anti-Human CD80 antibody monoclonal antibody (Catalog # HW630217 , Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

참고 문헌

데이터시트

Anti-Human CD80/B7-1 Antibody (SAA2207), FITC.pdf

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Anti-Human CD80/B7-1 Antibody (SAA2207), FITC