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概要
カタログ番号HG001818
説明
HEK293T Cell Line (HG001818) is a parental HEK293T cell line. Widely used as a host cell for transient transfection, stable cell line generation, and recombinant protein production.
Highlights
  • Parental Cell Line — Unmodified HEK293T cells suitable as host cells or negative controls.
  • High Transfection Efficiency — Contains the SV40 large T antigen for enhanced episomal replication of plasmids carrying the SV40 origin.
  • Versatile Applications — Ideal for transient transfection, lentiviral packaging, stable cell line development, and recombinant protein expression.
アプリケーションFCM
Human
宿主種 HEK293T
特性 Growth Properties: Adherent; Morphology: Epithelial; Culture Medium: DMEM + 10% FBS + 1% Penicillin-Streptomycin; Culture Conditions: 37°C, 5% CO₂
発送 Dry ice
安定性と保存 Frozen in liquid nitrogen.
仕様 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
別名293T, HEK-293T, HEK 293T
背景

HEK293T is a highly transfectable derivative of the human embryonic kidney 293 (HEK293) cell line that stably expresses the SV40 large T antigen. The presence of the T antigen allows episomal replication of transfected plasmids containing the SV40 origin of replication, leading to amplification of the plasmid and extended temporal expression of the desired gene products. HEK293T cells are widely used for transient protein expression, lentiviral and retroviral packaging, and as host cells for generating stable cell lines overexpressing specific target genes. They grow as an adherent monolayer and are readily maintained in standard culture conditions.

注意事項 For research use only
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