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Обзор
Каталожный номерHG001818
Описание
HEK293T Cell Line (HG001818) is a parental HEK293T cell line. Widely used as a host cell for transient transfection, stable cell line generation, and recombinant protein production.
Highlights
  • Parental Cell Line — Unmodified HEK293T cells suitable as host cells or negative controls.
  • High Transfection Efficiency — Contains the SV40 large T antigen for enhanced episomal replication of plasmids carrying the SV40 origin.
  • Versatile Applications — Ideal for transient transfection, lentiviral packaging, stable cell line development, and recombinant protein expression.
ПримененияFCM
ВидыHuman
Хозяинский вид HEK293T
Метод обнаружения Colorimetric
Тип образца Plasma, Serum
Тип анализа Quantitative
Диапазон 1.56 - 100 ng/mL
Чувствительность 0.25 ng/mL
Восстановление 80-120%
Свойства Growth Properties: Adherent; Morphology: Epithelial; Culture Medium: DMEM + 10% FBS + 1% Penicillin-Streptomycin; Culture Conditions: 37°C, 5% CO₂
Доставка Dry ice
Устойчивость и хранение Frozen in liquid nitrogen.
Характеристики 1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Альтернативные названия293T, HEK-293T, HEK 293T
Фон

HEK293T is a highly transfectable derivative of the human embryonic kidney 293 (HEK293) cell line that stably expresses the SV40 large T antigen. The presence of the T antigen allows episomal replication of transfected plasmids containing the SV40 origin of replication, leading to amplification of the plasmid and extended temporal expression of the desired gene products. HEK293T cells are widely used for transient protein expression, lentiviral and retroviral packaging, and as host cells for generating stable cell lines overexpressing specific target genes. They grow as an adherent monolayer and are readily maintained in standard culture conditions.

Примечание For research use only
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Ссылки
Даташит
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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