InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12) (HB822010)

데이터시트 제품 공유 정보 복사

가격(USD): $

사양:

100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호

HB822010

설명

InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12) [5D12] (HB822010) is a alpaca monoclonal antibody detecting CNR1 in ELISA, Neutralization. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, Neutralization, WB

숙주 종

Alpaca

이소타입

VHH-8His-Cys-tag

클론 ID

5D12

클론성

Monoclonal

테스트된 응용 분야

타겟

CNR1, CB1, CANN6, CB-R, CNR, Cannabinoid receptor 1

내독소 수준

<0.01 EU/ug

순도

>95% as determined by SDS-PAGE.

정제

Purified by Nickel column.

등록번호

P21554

RRID

InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12) (abinScience Cat# HB822010, RRID:AB_3727800)

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

배경

Cannabinoid receptor 1 (CNR1/CB1) is a ~52 kDa protein. G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP. In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses.

1. Ryberg, E. et al. (2005) FEBS letters 579, 259-64. PMID: 15620723

2. Hua, T. et al. (2016) Cell 167, 750-762.e14. PMID: 27768894

3. Shao, Z. et al. (2016) Nature 540, 602-606. PMID: 27851727

4. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350

5. Gérard, CM. et al. (1991) The Biochemical journal 279 ( Pt 1), 129-34. PMID: 1718258

6. Oddi, S. et al. (2012) British journal of pharmacology 165, 2635-51. PMID: 21895628

7. Niehaus, JL. et al. (2007) Molecular pharmacology 72, 1557-66. PMID: 17895407

8. Jourdan, T. et al. (2013) Nature medicine 19, 1132-40. PMID: 23955712

참고사항

For research use only. Not suitable for clinical or therapeutic use.

이미지

SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CNR1/CB1 Nanobody (5D12)
Bioactivity

Detects recombinant CNR1 (Catalog No: HB822012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CNR1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CNR1 (Catalog No: HB822012)