Research Grade Vopratelimab (HV702016)

데이터시트 제품 공유 정보 복사

가격(USD): $

사양:

100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호	HV702016
설명	Vopratelimab (HV702016) is a research-grade recombinant antibody targeting CD278. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
종 반응성	Human
응용 분야	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
숙주 종	Humanized
이소타입	IgG1-kappa
발현 시스템	Mammalian Cells
클론성	Monoclonal
타겟	CD278, ICOS, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM
내독소 수준	Please contact the lab for this information.
순도	>95% purity as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	Q9Y6W8
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
대체 이름	JTX-2011, 2039148-04-2
배경	Inducible T-cell costimulator (CD278/ICOS) is a ~22 kDa protein. Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. 1. Hutloff, A. et al. (1999) Nature 397, 263-6. PMID: 9930702 2. Rujas, E. et al. (2020) Nature communications 11, 5066. PMID: 33033255 3. Pedros, C. et al. (2016) Nature immunology 17, 825-33. PMID: 27135603 4. Wan, Z. et al. (2020) Cellular & molecular immunology 17, 143-152. PMID: 30523347
참고사항	For research use only. Not suitable for clinical or therapeutic use.

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Bioactivity

SEC-HPLC detection for Research Grade Vopratelimab.
SDS-PAGE

SDS-PAGE for Research Grade Vopratelimab.
Flow CytoMetry

Flow-cytometry using anti-human CD278 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD278 monoclonal antibody (Catalog: HV702016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD278/ICOS monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD278/ICOS (Catalog No: HV702011)
Lane 2: negative control