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| 카탈로그 번호 | HF004106 |
|---|---|
| 설명 |
Zatuximab (HF004106) is a research-grade recombinant antibody targeting HER1. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 숙주 종 | Chimeric |
| 이소타입 | IgG1-kappa |
| 발현 시스템 | Mammalian Cells |
| 클론성 | Monoclonal |
| 타겟 | HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB |
| 내독소 수준 | Please contact the lab for this information. |
| 순도 | >95% purity as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P00533 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS, pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| 대체 이름 | Futuximab, DS992, Sym004 (combination of modotuximab and futuximab), 1310460-86-6 |
| 배경 | Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix). 1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725 2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536 3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960 4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033 5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049 6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079 7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704 |
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
SEC-HPLC detection for Research Grade Zatuximab.
Detects EGFR/ERBB1/HER1 in indirect ELISAs.
SDS-PAGE for Research Grade Zatuximab
Flow-cytometry using APC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using FITC anti-human EGFR antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human EGFR monoclonal antibody (Catalog: HF004106, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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