Research Grade Anti-SARS-CoV-2 RBD antibody (EY6A) (VK565063)

데이터시트 제품 공유 정보 복사

가격(USD): $

사양:

20ug
50ug
100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트

개요

카탈로그 번호

VK565063

설명

Anti-SARS-CoV-2 RBD antibody (EY6A) (VK565063) is a research-grade recombinant antibody targeting Spike glycoprotein. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

종 반응성

SARS-CoV-2 (2019-nCoV)

응용 분야

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

숙주 종

Human

이소타입

IgG1

클론 ID

EY6A

발현 시스템

Mammalian Cells

클론성

Monoclonal

타겟

Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 2

내독소 수준

Please contact the lab for this information.

순도

>95% purity as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P0DTC2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

배경

Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

참고사항

For research use only. Not suitable for clinical or therapeutic use.

이미지

Bioactivity

Detects Recombinant SARS-CoV-2 Spike protein receptor binding domain (Cat No.: VK565021) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Research Grade Anti-SARS-CoV-2 RBD antibody (EY6A).