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  • 100ug
  • 1mg
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Обзор
Каталожный номерHB816026
Описание
Anti-Human oxMIF Reference Antibody (ON203, RUO) (HB816026) is a human monoclonal antibody detecting MIF,oxMIF.
Реактивность видовHuman
ПримененияELISA, FCM, Functional assay
Хозяинский видHuman
КлоональностьMonoclonal
ИзотипIgG1, kappa
ID клонаON203
Цель EC:5.3.2.1, EC:5.3.3.12, GIF, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, MIF, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Уровень эндотоксинов < 10 EU/mg as determined by LAL method.
Чистота >95% as determined by SDS-PAGE.
Очистка Protein A/G purified from cell culture supernatant.
Номер доступа P14174
Форма Liquid
Буфер хранения 0.01M PBS pH 7.4.

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Информация об использовании продукта
Применение Разбавление
ELISA, FACS, Functional assay
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term from the date of receipt..
Фон

Macrophage migration inhibitory factor (MIF/GIF) is a ~12 kDa protein. Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

1. Oddo, M. et al. (2005) Infection and immunity 73, 3783-6. PMID: 15908412
2. Emonts, M. et al. (2007) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 44, 1321-8. PMID: 17443469
3. Fan, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10994-9. PMID: 23776208
4. Tan, TH. et al. (2001) The Biochemical journal 357, 373-83. PMID: 11439086
Примечание For research use only. Not suitable for clinical or therapeutic use.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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