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| 카탈로그 번호 | HY353026 |
|---|---|
| 설명 |
Ganitumab (HY353026) is a research-grade recombinant antibody targeting CD221. Produced in mammalian cells with native-like glycosylation.
Highlights
|
| 종 반응성 | Human |
| 응용 분야 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 숙주 종 | Human |
| 이소타입 | IgG1-kappa |
| 발현 시스템 | Mammalian Cells |
| 클론성 | Monoclonal |
| 타겟 | CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor |
| 내독소 수준 | Please contact the lab for this information. |
| 순도 | >95% purity as determined by SDS-PAGE. |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | P08069 |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS, pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| 대체 이름 | AMG479, 905703-97-1 |
| 배경 | Insulin-like growth factor 1 receptor (CD221/IGF1R) is a ~154 kDa protein. Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. |
| 참고사항 | For research use only. Not suitable for clinical or therapeutic use. |
SEC-HPLC detection for Research Grade Ganitumab.
Flow-cytometry using anti-human IGFIR antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IGFIR monoclonal antibody (Catalog HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human IGFIR antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IGFIR monoclonal antibody (Catalog HY353026) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Research Grade Ganitumab
Flow-cytometry using APC anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human CD221 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD221 monoclonal antibody (Catalog: HY353026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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