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| 카탈로그 번호 | HC657022 |
|---|---|
| 설명 |
Recombinant Human GRIN2A Protein, N-His (HC657022) expressed in E. coli, spanning Pro401-Arg539. Purity: >90% by SDS-PAGE.
Highlights
|
| 발현 시스템 | E. coli |
| 등록번호 | Q12879 |
| 단백질 길이 | Pro401-Arg539 |
| 응용 분야 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 종 | Homo sapiens (Human) |
| 성질 | Recombinant |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >90% as determined by SDS-PAGE. |
| 예측 분자량 | 17.88 kDa |
| 형태 | Lyophilized |
| 저장 완충액 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 배송 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 대체 이름 | N-methyl D-aspartate receptor subtype 2A, NMDAR2A, Glutamate [NMDA] receptor subunit epsilon-1, hNR2A, NR2A, Glutamate receptor ionotropic, NMDA 2A, GRIN2A, GluN2A |
| 배경 | Glutamate receptor ionotropic, NMDA 2A (GRIN2A) is a ~165 kDa protein. Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). NMDARs participate in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators. 1. Endele, S. et al. (2010) Nature genetics 42, 1021-6. PMID: 20890276 2. Carvill, GL. et al. (2013) Nature genetics 45, 1073-6. PMID: 23933818 3. Lemke, JR. et al. (2013) Nature genetics 45, 1067-72. PMID: 23933819 4. Lesca, G. et al. (2013) Nature genetics 45, 1061-6. PMID: 23933820 5. Yuan, H. et al. (2014) Nature communications 5, 3251. PMID: 24504326 6. Hackos, DH. et al. (2016) Neuron 89, 983-99. PMID: 26875626 7. Volgraf, M. et al. (2016) Journal of medicinal chemistry 59, 2760-79. PMID: 26919761 8. Addis, L. et al. (2017) Scientific reports 7, 66. PMID: 28242877 9. Iacobucci, GJ. et al. (2022) Molecular psychiatry 27, 5113-5123. PMID: 36117210 10. Xu, Y. et al. (2024) Cellular and molecular life sciences : CMLS 81, 153. PMID: 38538865 |
| 참고사항 | For research use only. |
SDS-PAGE for Recombinant Human GRIN2A Protein, N-His
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