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Обзор
Каталожный номерMV606022
Описание
Recombinant Mouse LGMN Protein, N-His (MV606022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Система экспрессииE. coli
Номер доступаO89017
Длина белкаLys30-Tyr435
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыMus musculus (Mouse)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияEC:3.4.22.34, Legumain, Lgmn, Protease, cysteine 1, Prsc1
Фон

Legumain is a ~49 kDa protein. Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules.

1. Zhao, L. et al. (2014) Cell research 24, 344-58. PMID: 24407422
2. Chen, JM. et al. (1998) The Biochemical journal 335 ( Pt 1), 111-7. PMID: 9742219
3. Chen, JM. et al. (1998) FEBS letters 441, 361-5. PMID: 9891971
4. Rodríguez-Silvestre, P. et al. (2023) Science (New York, N.Y.) 380, 1258-1265. PMID: 37347855
5. Morita, Y. et al. (2007) FEBS letters 581, 1417-24. PMID: 17350006
6. Miller, G. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 1606-17. PMID: 21292981
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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