Mouse LGMN Recombinant Protein (N-His) (MV606012)

Name: Mouse LGMN Recombinant Protein (N-His)
Brand: abinScience
SKU: MV606012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MV606012
Description	Recombinant Mouse LGMN Protein, N-His (MV606012) expressed in E. coli, spanning Gly29-Met288. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O89017
Protein length	Gly29-Met288
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.94 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AEP, Asparaginyl endopeptidase, EC:3.4.22.34, LGMN, Legumain, PRSC1, Protease, cysteine 1
Background	Legumain is a ~49 kDa protein. Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. 1. Zhao, L. et al. (2014) Cell research 24, 344-58. PMID: 24407422 2. Chen, JM. et al. (1998) The Biochemical journal 335 ( Pt 1), 111-7. PMID: 9742219 3. Chen, JM. et al. (1998) FEBS letters 441, 361-5. PMID: 9891971 4. Rodríguez-Silvestre, P. et al. (2023) Science (New York, N.Y.) 380, 1258-1265. PMID: 37347855 5. Morita, Y. et al. (2007) FEBS letters 581, 1417-24. PMID: 17350006 6. Miller, G. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 1606-17. PMID: 21292981
Note	For research use only

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Mouse LGMN Recombinant Protein (N-His).pdf

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Mouse LGMN Recombinant Protein (N-His) (MV606012)

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