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| カタログ番号 | HF526066 |
|---|---|
| 種反応性 | Human |
| アプリケーション | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 宿主種 | Human |
| アイソタイプ | IgG1-kappa |
| クローンID | AMG 966 |
| 発現システム | Mammalian Cells |
| クローン性 | Monoclonal |
| ターゲット | TNFSF15, Vascular endothelial cell growth inhibitor, VEGI, TL1, TNF ligand-related molecule 1, Tumor necrosis factor ligand superfamily member 15, TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 |
| エンドトキシンレベル | Please contact the lab for this information. |
| 純度 | >95% purity as determined by SDS-PAGE. |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | O95150 & P01375 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS, pH 7.4. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| 別名 | Bispecific, AMG966, AMG-966 |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
SDS-PAGE for Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966).
Detects recombinant human TNFa (Cat No.: HF879011) in indirect ELISA.
Detects recombinant human TNFa (Cat No.: HF526011) in indirect ELISA.
Detects Recombinant Human TNFSF15/TL1A Protein, C-His (Cat No.: HF526011) in indirect ELISA.
Flow-cytometry APC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry FITC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF526066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-TNFSF15/TL1A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human TNFSF15/TL1A (Catalog No: HF526011)
Lane 2: negative control
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