Anti-ACTN4 Polyclonal Antibody (HT028014)

Name: Anti-ACTN4 Polyclonal Antibody
Brand: abinScience
SKU: HT028014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HT028014

Description

Anti-ACTN4 Polyclonal Antibody (HT028014) is a rabbit polyclonal antibody detecting ACTN4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human ACTN4 (Tyr611-Leu911).

Cible

Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

O43707

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Alpha-actinin-4 (ACTN4) is a ~104 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2.

1. Yan, Q. et al. (2005) Molecular biology of the cell 16, 2470-82. PMID: 15772161

2. Khurana, S. et al. (2012) The Journal of biological chemistry 287, 12027-35. PMID: 22351778

3. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACTN4 polyclonal antibody at ACTN4 on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A431 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse skeletal muscle lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate

Reférences

Fiche technique

Anti-ACTN4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-ACTN4 Polyclonal Antibody (HT028014)

Aperçu

Images

Reférences

Fiche technique

SDS