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Anti-Human ACTN4/Actinin alpha 4 Recombinant Antibody (SAA1752) (HT028013)

Anti-Human ACTN4/Actinin alpha 4 Recombinant Antibody (SAA1752)
Anti-Human ACTN4/Actinin alpha 4 Recombinant Antibody (SAA1752)
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Overview
Catalog No.HT028013
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1752
Clonality Monoclonal
Target Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession O43707
RRID Anti-Human ACTN4/Actinin alpha 4 Antibody (SAA1752) (abinScience Cat# HT028013, RRID:AB_3727513)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Alpha-actinin-4 (ACTN4) is a ~104 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2.

1. Yan, Q. et al. (2005) Molecular biology of the cell 16, 2470-82. PMID: 15772161
2. Khurana, S. et al. (2012) The Journal of biological chemistry 287, 12027-35. PMID: 22351778
3. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882
Note For research use only.
Images
  • Anti-Human ACTN4/Actinin alpha 4 Recombinant Antibody (SAA1752)

    SDS-PAGE

    SDS-PAGE for Anti-Human ACTN4/Actinin alpha 4 Antibody (SAA1752)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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