Anti-ACTN4 Polyclonal Antibody (HT028014)

Name: Anti-ACTN4 Polyclonal Antibody
Brand: abinScience
SKU: HT028014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HT028014

説明

Anti-ACTN4 Polyclonal Antibody (HT028014) is a rabbit polyclonal antibody detecting ACTN4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human ACTN4 (Tyr611-Leu911).

ターゲット

Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

O43707

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Alpha-actinin-4 (ACTN4) is a ~104 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2.

1. Yan, Q. et al. (2005) Molecular biology of the cell 16, 2470-82. PMID: 15772161

2. Khurana, S. et al. (2012) The Journal of biological chemistry 287, 12027-35. PMID: 22351778

3. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882

注意事項

For research use only.

イメージ

Western blot

Western blot analysis was performed using anti-ACTN4 polyclonal antibody at ACTN4 on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A431 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse skeletal muscle lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate

参考文献

データシート

Anti-ACTN4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-ACTN4 Polyclonal Antibody (HT028014)

概要

イメージ

参考文献

データシート

SDS