Anti-ACTN4 Polyclonal Antibody (HT028014)

Name: Anti-ACTN4 Polyclonal Antibody
Brand: abinScience
SKU: HT028014
Price: 96 USD
Availability: InStock

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Каталожный номер

HT028014

Описание

Anti-ACTN4 Polyclonal Antibody (HT028014) is a rabbit polyclonal antibody detecting ACTN4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human ACTN4 (Tyr611-Leu911).

Цель

Non-muscle alpha-actinin 4, Alpha-actinin-4, ACTN4

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

O43707

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Alpha-actinin-4 (ACTN4) is a ~104 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2.

1. Yan, Q. et al. (2005) Molecular biology of the cell 16, 2470-82. PMID: 15772161

2. Khurana, S. et al. (2012) The Journal of biological chemistry 287, 12027-35. PMID: 22351778

3. Gordón-Alonso, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 689-700. PMID: 22689882

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-ACTN4 polyclonal antibody at ACTN4 on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A431 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse heart lysate
Lane 8: Mouse skeletal muscle lysate
Lane 9: Mouse kidney lysate
Lane 10: Rat brain lysate

Ссылки

Даташит

Anti-ACTN4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-ACTN4 Polyclonal Antibody (HT028014)

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Изображения

Ссылки

Даташит

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