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Anti-AKT2 Polyclonal Antibody (HB933014)

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Número de catálogoHB933014
Descripción
Anti-AKT2 Polyclonal Antibody (HB933014) is a rabbit polyclonal antibody detecting AKT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human AKT2 (Met1-Lys146).
Objetivo PKB beta, Protein kinase Akt-2, AKT2, Protein kinase B beta, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
Purificación Purified by antigen affinity column.
Número de acceso P31751
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

RAC-beta serine/threonine-protein kinase (AKT2) is a ~55 kDa protein. Serine/threonine kinase closely related to AKT1 and AKT3. All 3 enzymes, AKT1, AKT2 and AKT3, are collectively known as AKT kinase. AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis, through the phosphorylation of a range of downstream substrates. Over 100 substrates have been reported so far, although for most of them, the precise AKT kinase catalyzing the reaction was not specified. AKT regulates glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface.

1. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781
2. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960
3. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173
4. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369
Nota For research use only.
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Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AKT2 Polyclonal Antibody

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