Human AKT2 Recombinant Protein (N-His) (HB933012)

Name: Human AKT2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB933012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB933012
Description	Recombinant Human AKT2 Protein, N-His (HB933012) expressed in E. coli, spanning Met1-Lys146. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P31751
Protein length	Met1-Lys146
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PKB beta, Protein kinase Akt-2, AKT2, Protein kinase B beta, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
Background	RAC-beta serine/threonine-protein kinase (AKT2) is a ~55 kDa protein. Serine/threonine kinase closely related to AKT1 and AKT3. All 3 enzymes, AKT1, AKT2 and AKT3, are collectively known as AKT kinase. AKT regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis, through the phosphorylation of a range of downstream substrates. Over 100 substrates have been reported so far, although for most of them, the precise AKT kinase catalyzing the reaction was not specified. AKT regulates glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. 1. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781 2. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960 3. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173 4. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369
Note	For research use only.

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SDS-PAGE for Recombinant Human AKT2 Protein, N-His

References

HB933012-SDS-PAGE-1.jpg

Datasheet

Human AKT2 Recombinant Protein (N-His).pdf

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Human AKT2 Recombinant Protein (N-His) (HB933012)

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