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Anti-AP2B1 Polyclonal Antibody
Anti-AP2B1 Polyclonal Antibody
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Спецификация:
  • 20ug
  • 50ug
  • 100ug
Количество:
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Обзор
Каталожный номерHX073014
Описание
Anti-AP2B1 Polyclonal Antibody (HX073014) is a rabbit polyclonal antibody detecting AP2B1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human AP2B1 (Met1-Phe266).
Цель Adaptor-related protein complex 2 subunit beta, ADTB2, CLAPB1, Beta-adaptin, AP105B, AP-2 complex subunit beta, Plasma membrane adaptor HA2/AP2 adaptin beta subunit, AP2B1, Adaptor protein complex AP-2 subunit beta, Clathrin assembly protein complex 2 beta large chain, Beta-2-adaptin
Очистка Purified by antigen affinity column.
Номер доступа P63010
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

AP-2 complex subunit beta (AP2B1) is a ~104 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.

1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
Примечание For research use only.
Изображения
  • Anti-AP2B1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AP2B1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse cerebellum lysate
    Lane 3: Mouse brain lysate
    Lane 4: Rat cerebellum lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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