Human AP2B1 Recombinant Protein (N-His) (HX073012)

Name: Human AP2B1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX073012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX073012
Description	Recombinant Human AP2B1 Protein, N-His (HX073012) expressed in E. coli, spanning Met1-Phe266. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P63010
Protein length	Met1-Phe266
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.04 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Adaptor-related protein complex 2 subunit beta, ADTB2, CLAPB1, Beta-adaptin, AP105B, AP-2 complex subunit beta, Plasma membrane adaptor HA2/AP2 adaptin beta subunit, AP2B1, Adaptor protein complex AP-2 subunit beta, Clathrin assembly protein complex 2 beta large chain, Beta-2-adaptin
Background	AP-2 complex subunit beta (AP2B1) is a ~104 kDa protein. Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. 1. Marcello, E. et al. (2013) The Journal of clinical investigation 123, 2523-38. PMID: 23676497
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human AP2B1 Protein, N-His

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Datasheet

Human AP2B1 Recombinant Protein (N-His).pdf

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Formula

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Human AP2B1 Recombinant Protein (N-His) (HX073012)

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