Anti-ARIH2 Polyclonal Antibody (HD428014)

Name: Anti-ARIH2 Polyclonal Antibody
Brand: abinScience
SKU: HD428014
Price: 96 USD
Availability: InStock

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20ug
50ug
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Número de catálogo

HD428014

Descripción

Anti-ARIH2 Polyclonal Antibody (HD428014) is a rabbit polyclonal antibody detecting ARIH2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human ARIH2 (Thr62-Thr493).

Objetivo

ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

O95376

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

O95376

Fondo

E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB.

1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314

2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546

3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006

4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685

7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-ARIH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate
Lane 5: Hela cell lysate

Referencias

Datasheet

Anti-ARIH2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-ARIH2 Polyclonal Antibody (HD428014)

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Referencias

Datasheet

SDS